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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAMC1 Monoclonal antibody

LAMC1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G2G4

Cat no : 67706-1-Ig

Synonyms

LAMB2, LAMC1, Laminin 1 subunit gamma, Laminin 10 subunit gamma, Laminin 11 subunit gamma, Laminin 2 subunit gamma, Laminin 3 subunit gamma, Laminin 4 subunit gamma, Laminin 6 subunit gamma, Laminin 7 subunit gamma, Laminin 8 subunit gamma, Laminin 9 subunit gamma, Laminin B2 chain, Laminin subunit gamma 1, S LAM gamma, S laminin subunit gamma

Validation Data Gallery

Applications:
  • WB analysis using 67706-1-Ig

    WB analysis using 67706-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 67706-1-Ig

    WB analysis of human placenta using 67706-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 67706-1-Ig

    WB analysis of LNCaP using 67706-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat rectum using 67706-1-Ig

    WB analysis of rat rectum using 67706-1-Ig

    rat rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig rectum using 67706-1-Ig

    WB analysis of pig rectum using 67706-1-Ig

    pig rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67706-1-Ig

    IF Staining of HeLa using 67706-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LAMC1 antibody (67706-1-Ig, Clone: 1G2G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue, LNCaP cells, rat rectum tissue, pig rectum tissue, HepG2 cells, JAR cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

67706-1-Ig targets LAMC1 in WB, IF, ELISA applications and shows reactivity with Human, Pig, Rat samples.

Tested Reactivity Human, Pig, Rat
Cited Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LAMC1 fusion protein Ag14674 相同性解析による交差性が予測される生物種
Full Name laminin, gamma 1 (formerly LAMB2)
Calculated molecular weight 178 kDa
Observed molecular weight200-220 kDa
GenBank accession numberBC015586
Gene symbol LAMC1
Gene ID (NCBI) 3915
RRIDAB_2882897
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LAMC1 antibody 67706-1-IgDownload protocol
IF protocol for LAMC1 antibody 67706-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neuroscience

Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety.

Authors - Dan Liu
View Article
humanWB

Rheumatology (Oxford)

The central helicase domain holds the major conformational epitopes of melanoma differentiation-associated gene 5 autoantibodies

Authors - Yongxin Mo
View Article