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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated L2HGDH Monoclonal antibody

L2HGDH Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D11C2

Cat no : CL594-66269

Synonyms

C14orf160, DURANIN, FLJ12618, L2HGDH

Validation Data Gallery

Applications:
  • IF Staining of human breast cancer using CL594-66269

    IF Staining of human breast cancer using CL594-66269

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 L2HGDH antibody (CL594-66269, Clone: 3D11C2 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman breast cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 66269-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66269 targets L2HGDH in IF applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen L2HGDH fusion protein Ag8382 相同性解析による交差性が予測される生物種
Full Name L-2-hydroxyglutarate dehydrogenase
Calculated molecular weight463aa,50 kDa; 441aa,49 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC006117
Gene symbol L2HGDH
Gene ID (NCBI) 79944
RRIDAB_2919974
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.

Protocols

Product Specific Protocols
IF protocol for CL594 L2HGDH antibody CL594-66269Download protocol
Standard Protocols
Click here to view our Standard Protocols