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Cytokeratin 15 抗体 0 文献

Mouse Monoclonal| カタログ番号: 60247-1-Ig |クローン番号: 6B4F8

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適用アプリケーション

アプリケーション:
WB, IHC, IF, FC,ELISA
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Images See all

Cytokeratin 15 Antibody WB A431 cells 60247-1-Ig

A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cytokeratin 15 Antibody IHC human oesophagus cancer tissue 60247-1-Ig

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens).

Cytokeratin 15 Antibody IHC human oesophagus cancer tissue 60247-1-Ig

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens).

Cytokeratin 15 Antibody IF HeLa cells 60247-1-Ig

Immunofluorescent analysis of HeLa cells using 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Cytokeratin 15 Antibody FC A431 cells 60247-1-Ig

1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 15 (60247-1-Ig, Clone:6B4F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Samples were fixed with 90% MeOH.

種特異性:
human, mouse
WB ポジティブ:
A431 cells
IHC ポジティブ:
human oesophagus cancer tissue, human skin cancer tissue
IF ポジティブ:
HeLa cells
FC ポジティブ:
A431 cells
推奨希釈倍率:
WB : 1:500-1:2000
IHC : 1:100-1:1000
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein G purification
アイソタイプ:
IgG1
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
Cytokeratin 15 fusion protein Ag0185
正式名:
keratin 15
計算分子量:
49 kDa
検出分子量:
49 kDa
GenBank accession number:
BC002641
Gene ID (NCBI):
3866
遺伝子シンボル:
KRT15
別名:
CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

背景説明

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.


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