Validation Data Gallery

Filter: AllWBIPIHCIF/ICCIFFC (Intra)

WB analysis of A431 using 10143-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat skin using 10143-1-AP
rat skin tissue were subjected to SDS PAGE followed by western blot with 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 10143-1-AP
IP result of anti-Cytokeratin 14 (IP:10143-1-AP, 3ug; Detection:10143-1-AP 1:1000) with A431 cells lysate 1200ug.
IHC staining of human lung cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human lung cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:400 (under 10x lens).
IHC staining of human skin using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:400 (under 40x lens).
IHC staining of human skin cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human oesophagus cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 10143-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 14 antibody (10143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HaCaT using 10143-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 14 antibody (10143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10143-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse olfactory epithelium tissue using 10143-1-AP
IF result of CK14 (10143-1-AP, 1:400) with 1% PLP fixed basal quiescent stem cells of the adult mouse olfactory epithelium. (Green: CK14; Blue: DAPI). Basal lamina is marked by an arrow head. By Brian Lin, Tufts University.
FC experiment of A431 using 10143-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 14 (10143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10143-1-AP

Publications(98)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

KRT14, CK 14, CK14, CK-14, Cytokeratin,Keratin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (98)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, rat skin tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue, human cervical cancer tissue, human oesophagus cancer tissue, human skin tissue, human skin cancer tissue, mouse skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HaCaT cells, HeLa cells, HepG2 cells, mouse olfactory epithelium tissue
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 16 publications below
IHC	See 26 publications below
IF	See 59 publications below

Product Information

10143-1-AP targets Cytokeratin 14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0188 Product name: Recombinant human KRT14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 262-472 aa of BC002690 Sequence: GQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN 相同性解析による交差性が予測される生物種
Full Name	keratin 14
Calculated molecular weight	472 aa, 52 kDa
Observed molecular weight	47-50 kDa
GenBank accession number	BC002690
Gene Symbol	Cytokeratin 14
Gene ID (NCBI)	3861
RRID	AB_2134831
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02533
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Protocols

Product Specific Protocols
IF protocol for Cytokeratin 14 antibody 10143-1-AP	Download protocol
IHC protocol for Cytokeratin 14 antibody 10143-1-AP	Download protocol
IP protocol for Cytokeratin 14 antibody 10143-1-AP	Download protocol
WB protocol for Cytokeratin 14 antibody 10143-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Cell Stem Cell Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency. Authors - Brian Lin View Article
mouse	IF	Adv Sci (Weinh) Photothermal-Responsive Soluble Microneedle Patches for Meibomian Gland Dysfunction Therapy Authors - Fei Yu View Article
mouse	IF	Nat Commun Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica. Authors - Yue Hong View Article
human	IF	Nat Commun Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure. Authors - Vlasta Lungova View Article
mouse	IF	Biomaterials MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system. Authors - Tian Wang View Article
human	WB	Oncogene EB-virus latent membrane protein 1 potentiates the stemness of nasopharyngeal carcinoma via preferential activation of PI3K/AKT pathway by a positive feedback loop. Authors - C-F Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 10143-1-AP

WB analysis of rat skin using 10143-1-AP

IP experiment of A431 using 10143-1-AP

IHC staining of human lung cancer using 10143-1-AP

IHC staining of human lung cancer using 10143-1-AP

IHC staining of human lung cancer using 10143-1-AP

IHC staining of human lung cancer using 10143-1-AP

IHC staining of human cervical cancer using 10143-1-AP

IHC staining of human cervical cancer using 10143-1-AP

IHC staining of mouse skin using 10143-1-AP

IHC staining of mouse skin using 10143-1-AP

IHC staining of human skin using 10143-1-AP

IHC staining of human skin using 10143-1-AP

IHC staining of human skin cancer using 10143-1-AP

IHC staining of human skin cancer using 10143-1-AP

IHC staining of human cervical cancer using 10143-1-AP

IHC staining of human cervical cancer using 10143-1-AP

IHC staining of human breast cancer using 10143-1-AP

IHC staining of human breast cancer using 10143-1-AP

IHC staining of human oesophagus cancer using 10143-1-AP

IHC staining of human oesophagus cancer using 10143-1-AP

IF Staining of HepG2 using 10143-1-AP

IF Staining of HaCaT using 10143-1-AP

IF Staining of HeLa using 10143-1-AP

IF Staining of mouse olfactory epithelium tissue using 10143-1-AP

FC experiment of A431 using 10143-1-AP

Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Photothermal-Responsive Soluble Microneedle Patches for Meibomian Gland Dysfunction Therapy

Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica.

Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure.

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

EB-virus latent membrane protein 1 potentiates the stemness of nasopharyngeal carcinoma via preferential activation of PI3K/AKT pathway by a positive feedback loop.