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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLK11 Monoclonal antibody

KLK11 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E2B4

Cat no : 67427-1-Ig

Synonyms

Hippostasin, hK11, Kallikrein 11, KLK11, PRSS20, Serine protease 20, TLSP, Trypsin like protease

Validation Data Gallery

Applications:
  • WB analysis using 67427-1-Ig

    WB analysis using 67427-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67427-1-Ig

    WB analysis using 67427-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67427-1-Ig

    IHC staining of human breast cancer using 67427-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IHC staining of human breast cancer using 67427-1-Ig

    IHC staining of human breast cancer using 67427-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IHC staining of human prostate cancer using 67427-1-Ig

    IHC staining of human prostate cancer using 67427-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IHC staining of human prostate cancer using 67427-1-Ig

    IHC staining of human prostate cancer using 67427-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IF Staining of human breast cancer using 67427-1-Ig

    IF Staining of human breast cancer using 67427-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using KLK11 antibody (67427-1-Ig, Clone: 1E2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human breast cancer using 67427-1-Ig

    IF Staining of human breast cancer using 67427-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using KLK11 antibody (67427-1-Ig, Clone: 1E2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inDU 145 cells, pig brain tissue, LNCaP cells, PC-3 cells, Jurkat cells, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy KLK11 Ready-To-Use IHC Kit. KLK11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67427-1-Ig targets KLK11 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Rat samples.

Tested Reactivity Human, Pig, Rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KLK11 fusion protein Ag29895 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 11
Calculated molecular weight 250 aa, 27 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC022068
Gene symbol KLK11
Gene ID (NCBI) 11012
RRIDAB_2882666
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

Protocols

Product Specific Protocols
WB protocol for KLK11 antibody 67427-1-IgDownload protocol
IHC protocol for KLK11 antibody 67427-1-IgDownload protocol
IF protocol for KLK11 antibody 67427-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Bioenerg Biomembr

KLK11 acts as a tumor-inhibitor in laryngeal squamous cell carcinoma through the inactivation of Akt/Wnt/β-catenin signaling.

Authors - Ruimin Zhao
View Article
humanWB,IHC

Carcinogenesis

miR-1304 targets KLK11 to regulate gastric cancer cell proliferation through the mTOR signaling pathway

Authors - Yi Ding
View Article