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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

JNK Recombinant antibody

JNK Recombinant Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6J16

Cat no : 81629-1-RR

Synonyms

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

Validation Data Gallery

Applications:
  • WB analysis using 81629-1-RR

    WB analysis using 81629-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81629-1-RR (JNK antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 81629-1-RR

    IHC staining of human colon cancer using 81629-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 81629-1-RR

    IHC staining of human colon cancer using 81629-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 81629-1-RR

    IF Staining of Jurkat using 81629-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 81629-1-RR

    IF Staining of HeLa using 81629-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 81629-1-RR

    FC experiment of HeLa using 81629-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human JNK (81629-1-RR, Clone:6J16) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, Jurkat cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

81629-1-RR targets JNK in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen JNK fusion protein Ag1639 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 8
Calculated molecular weight 48 kDa
Observed molecular weight44-48 kDa, 50-55 kDa
GenBank accession numberBC130572
Gene symbol MAPK8
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has some isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ). This antibody can recognize JNK1, JNK2 and JNK3.

Protocols

Product Specific Protocols
WB protocol for JNK antibody 81629-1-RRDownload protocol
IHC protocol for JNK antibody 81629-1-RRDownload protocol
IF protocol for JNK antibody 81629-1-RRDownload protocol
FC protocol for JNK antibody 81629-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols