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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated ITM2C Monoclonal antibody

ITM2C Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, canine

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E8G11

Cat no : CL594-60095

Synonyms

BRI3, BRICD2C, Cerebral protein 14, E25, E25C, integral membrane protein 2C, ITM2C, ITM3, PSEC0047, Transmembrane protein BRI3

Validation Data Gallery

Applications:
  • IF Staining of MDCK using CL594-60095

    IF Staining of MDCK using CL594-60095

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using the CoraLite®594-conjugated version of this antibody, CL594-60095 (ITM2C antibody), at dilution of 1:100.

  • IF Staining of HepG2 using CL594-60095

    IF Staining of HepG2 using CL594-60095

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60095 (ITM2C antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inMDCK cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60095-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60095 targets ITM2C in IF applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ITM2C fusion protein Ag4409 相同性解析による交差性が予測される生物種
Full Name integral membrane protein 2C
Calculated molecular weight230aa,26 kDa; 267aa,30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC025742
Gene symbol ITM2C
Gene ID (NCBI) 81618
RRIDAB_2883421
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Integral membrane protein 2C (ITM2C) is a type II integral transmembrane protein belonging to a family composed of at least two other members, ITM2A and ITM2B. ITM2C, often called transmembrane protein BRI3, is highly expressed in brain tissue. Yeast two-hybrid screen system revealed that BRI3 could interact with beta-secretase beta-amyloid protein converting enzyme (BACE)1 and the microtubule-destabilizing protein SCG10 (STMN2). It may play a role in TNF-induced cell death and neuronal differentiation. BRI3 was found to inhibit the various processing of amyloid precursor protein (APP) by blocking the access of alpha- and beta-secretases to APP, competitive inhibition of APP processing by BRI3 may provide a new approach to Alzheimer disease (AD) therapy and prevention.

Protocols

Product Specific Protocols
IF protocol for CL594 ITM2C antibody CL594-60095Download protocol
Standard Protocols
Click here to view our Standard Protocols