ITIH3 Polyclonal antibody
ITIH3 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21247-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | human serum tissue |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
The immunogen of 21247-1-AP is ITIH3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ITIH3 fusion protein Ag15750 相同性解析による交差性が予測される生物種 |
| Full Name | inter-alpha (globulin) inhibitor H3 |
| Calculated molecular weight | 890 aa, 100 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC107604 |
| Gene symbol | ITIH3 |
| Gene ID (NCBI) | 3699 |
| RRID | AB_2878830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ITIH3 antibody 21247-1-AP | Download protocol |
| IHC protocol for ITIH3 antibody 21247-1-AP | Download protocol |
| IP protocol for ITIH3 antibody 21247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. | ||
J Pers Med Comprehensive Analysis and Experimental Validation of a Novel Estrogen/Progesterone-Related Prognostic Signature for Endometrial Cancer. | ||
Dis Markers Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer. |
