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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ISG15 Monoclonal antibody

ISG15 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G9A2

Cat no : 68408-1-Ig

Synonyms

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

Validation Data Gallery

Applications:
  • WB analysis using 68408-1-Ig

    WB analysis using 68408-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68408-1-Ig

    WB analysis of A431 using 68408-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68408-1-Ig

    WB analysis using 68408-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMDA-MB-231 cells, LNCaP cells, A431 cells, HeLa cells, HCT 116 cells, HeLa cells, DU 145 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

68408-1-Ig targets ISG15 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ISG15 fusion protein Ag8953 相同性解析による交差性が予測される生物種
Full Name ISG15 ubiquitin-like modifier
Calculated molecular weight 165 aa, 18 kDa
Observed molecular weight 13-17 kDa
GenBank accession numberBC009507
Gene symbol ISG15
Gene ID (NCBI) 9636
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

Protocols

Product Specific Protocols
WB protocol for ISG15 antibody 68408-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols