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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated INS Polyclonal antibody

INS Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-15848

Synonyms

ILPR, INS, insulin, IRDN

Validation Data Gallery

Applications:
  • IF Staining of mouse pancreas using CL594-15848

    IF Staining of mouse pancreas using CL594-15848

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 INS antibody (CL594-15848) at dilution of 1:200, CoraLite®488 Glucagon antibody (CL488-15954, green).

  • IF Staining of mouse pancreas using CL594-15848

    IF Staining of mouse pancreas using CL594-15848

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 INS antibody (CL594-15848) at dilution of 1:200, CoraLite®488 Glucagon antibody (CL488-15954, green).

  • IF Staining of human pancreas cancer using CL594-15848

    IF Staining of human pancreas cancer using CL594-15848

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CoraLite®594 INS antibody (CL594-15848) at dilution of 1:200.

  • IF Staining of human pancreas cancer using CL594-15848

    IF Staining of human pancreas cancer using CL594-15848

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CoraLite®594 INS antibody (CL594-15848) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse pancreas tissue, human pancreas cancer tissue
Planning an IHC experiment? We recommend our IHCeasy Insulin Ready-To-Use IHC Kit. Insulin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15848-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

CL594-15848 targets INS in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INS fusion protein Ag8630 相同性解析による交差性が予測される生物種
Full Name INS
Calculated molecular weight 110 aa, 12 kDa
GenBank accession numberBC005255
Gene symbol INS
Gene ID (NCBI) 3630
RRIDAB_2919824
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus.

Protocols

Product Specific Protocols
IF protocol for CL594 INS antibody CL594-15848Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Food Funct

Lycopene ameliorates islet function and down-regulates the TLR4/MyD88/NF-κB pathway in diabetic mice and Min6 cells

Authors - Yage Liu
View Article