CoraLite® Plus 488-conjugated IL-33 Monoclonal antibody
IL-33 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgM
Reactivity
human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A9C7
Cat no : CL488-66235
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66235-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66235 targets IL-33 in IF applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IL-33 fusion protein Ag21430 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 33 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC047085 |
| Gene symbol | IL33 |
| Gene ID (NCBI) | 90865 |
| RRID | AB_2883291 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 IL-33 antibody CL488-66235 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
