IL-27 Monoclonal antibody
IL-27 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IHC
Conjugate
Unconjugated
CloneNo.
1E1H5
Cat no : 66164-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein, Ag21436 |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IHC | See 1 publications below |
Product Information
66164-1-Ig targets IL-27 in WB, IHC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IL-27 fusion protein Ag21436 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 27 |
| Calculated molecular weight | 243 aa, 27 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC062422 |
| Gene symbol | IL27 |
| Gene ID (NCBI) | 246778 |
| RRID | AB_2881560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-27 antibody 66164-1-Ig | Download protocol |
| IHC protocol for IL-27 antibody 66164-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Anticancer Res The Prognostic Significance of Peritumoral Lymphocytes' Band-like Structure in Type II Endometrial Cancer. |
