IL-1 Beta Recombinant antibody
IL-1 Beta Recombinant Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB,ELISA
Conjugate
Unconjugated
CloneNo.
3I9
Cat no : 82696-6-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LPS and Protein transport inhibitor treated THP-1 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Sample-dependent, check data in validation data gallery |
Product Information
82696-6-RR targets IL-1 Beta as part of a matched antibody pair:
MP80001-1: 82696-6-RR capture and 65276-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
Full Name | interleukin 1, beta |
Calculated molecular weight | 269 aa, 31 kDa |
Observed molecular weight | 30-35 kDa |
GenBank accession number | BC008678 |
Gene symbol | IL1B |
Gene ID (NCBI) | 3553 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.
Protocols
Product Specific Protocols | |
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WB protocol for IL-1 Beta antibody 82696-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |