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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-12 p70 Polyclonal antibody

IL-12 p70 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30756-1-AP

Synonyms

IL12, IL-12, IL-12 p70

Validation Data Gallery

Applications:
  • WB analysis using 30756-1-AP

    WB analysis using 30756-1-AP

    Recombinant protein Eg0742 (IL-12 p70) was subjected to SDS PAGE followed by western blot with 30756-1-AP (IL12(IL12B+IL12A) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30756-1-AP

    WB analysis using 30756-1-AP

    Recombinant protein Eg0299 (IL-12 P40) was subjected to SDS PAGE followed by western blot with 30756-1-AP (IL12(IL12B+IL12A) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

30756-1-AP targets IL-12 p70 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 12b
Calculated molecular weight70 kDa
Observed molecular weight 70 kDa
Gene symbol Il12b
Gene ID (NCBI) 16160
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.

Protocols

Product Specific Protocols
WB protocol for IL-12 p70 antibody 30756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols