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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human IL-7 (1C11B11)

IL-7 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

1C11B11

Cat no : PE-65281

Synonyms

IL 7, IL7, interleukin 7

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using PE-65281

    FC experiment of human PBMCs using PE-65281

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug PE Anti-Human IL-7 (PE-65281, Clone:1C11B11) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using PE-65281

    FC experiment of human PBMCs using PE-65281

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug PE Anti-Human IL-7 (PE-65281, Clone:1C11B11) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug PE Anti-Human IL-7 (PE-65281, Clone1C11B11) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

Tested Applications

Positive FC detected inLPS and Brefeldin A treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

PE-65281 targets IL-7 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-7 fusion protein HZ-1281 相同性解析による交差性が予測される生物種
Full Name interleukin 7
Calculated molecular weight 20 kDa
GenBank accession numberBC047698
Gene symbol IL7
Gene ID (NCBI) 3574
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)

Protocols

Product Specific Protocols
FC protocol for PE IL-7 antibody PE-65281Download protocol
Standard Protocols
Click here to view our Standard Protocols