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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HELIOS Monoclonal antibody

HELIOS Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F1F10

Cat no : 68558-1-Ig

Synonyms

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 68558-1-Ig

    WB analysis of Jurkat using 68558-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MOLT-4 using 68558-1-Ig

    WB analysis of MOLT-4 using 68558-1-Ig

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (IKZF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 68558-1-Ig

    IP experiment of Jurkat using 68558-1-Ig

    IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug.

Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

68558-1-Ig targets HELIOS in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HELIOS fusion protein Ag4357 相同性解析による交差性が予測される生物種
Full Name IKAROS family zinc finger 2 (Helios)
Calculated molecular weight 500 aa, 57 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC028936
Gene symbol IKZF2
Gene ID (NCBI) 22807
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).

Protocols

Product Specific Protocols
WB protocol for HELIOS antibody 68558-1-IgDownload protocol
IP protocol for HELIOS antibody 68558-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols