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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IgG Kappa Light Chain Polyclonal antibody

IgG Kappa Light Chain Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14678-1-AP

Synonyms

IgG Kappa Light Chain, IGK, IGK-, IGKC, immunoglobulin kappa locus

Validation Data Gallery

Applications:
  • WB analysis of human plasma using 14678-1-AP

    WB analysis of human plasma using 14678-1-AP

    human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 14678-1-AP

    WB analysis of human liver using 14678-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 14678-1-AP

    WB analysis of mouse skeletal muscle using 14678-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 14678-1-AP

    IHC staining of human spleen using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 14678-1-AP

    IHC staining of human spleen using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of mouse spleen using 14678-1-AP

    IHC staining of mouse spleen using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse spleen using 14678-1-AP

    IHC staining of mouse spleen using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 14678-1-AP

    IHC staining of human heart using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human heart using 14678-1-AP

    IHC staining of human heart using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human placenta using 14678-1-AP

    IHC staining of human placenta using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human placenta using 14678-1-AP

    IHC staining of human placenta using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human testis using 14678-1-AP

    IHC staining of human testis using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 14678-1-AP

    IHC staining of human testis using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human skin using 14678-1-AP

    IHC staining of human skin using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 14678-1-AP

    IHC staining of human skin using 14678-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inhuman plasma tissue, human liver tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman spleen tissue, human heart tissue, human placenta tissue, human skin tissue, human testis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14678-1-AP targets IgG Kappa Light Chain in WB, RIP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IgG Kappa Light Chain fusion protein Ag6389 相同性解析による交差性が予測される生物種
Full Name immunoglobulin kappa locus
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC030813
Gene symbol IGK-
Gene ID (NCBI) 50802
RRIDAB_1851235
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the light chain (Kappa) of human IgG.

Protocols

Product Specific Protocols
WB protocol for IgG Kappa Light Chain antibody 14678-1-APDownload protocol
IHC protocol for IgG Kappa Light Chain antibody 14678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Nat Commun

A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.

Authors - Christine Quach
View Article
IHC

PLoS Genet

Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis.

Authors - Hyun-Jin Na
View Article
humanRIP

J Bone Miner Metab

Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes.

Authors - Lanjuan Shen
View Article
mouseRIP

Mol Med Rep

Long non‑coding RNA XIST promotes cerebral ischemia/reperfusion injury by modulating miR‑27a‑3p/FOXO3 signaling.

Authors - Hua Zhang
View Article
mouseRIP

J Exp Clin Cancer Res

RNA N6-methyladenosine reader IGF2BP2 promotes lymphatic metastasis and epithelial-mesenchymal transition of head and neck squamous carcinoma cells via stabilizing slug mRNA in an m6A-dependent manner.

Authors - Dan Yu
View Article
humanIF

EBioMedicine

Integrating microarray-based spatial transcriptomics and single-cell RNA-sequencing reveals tissue architecture in esophageal squamous cell carcinoma

Authors - Wei Guo
View Article