CoraLite® Plus 488-conjugated IDE Monoclonal antibody
IDE Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3E10G1
Cat no : CL488-67106
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | A549 cells |
Positive FC detected in | A549 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-67106 targets IDE in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | IDE fusion protein Ag17161 相同性解析による交差性が予測される生物種 |
Full Name | Abeta degrading protease |
Calculated molecular weight | 1019 aa, 118 kDa |
Observed molecular weight | 100-120 kDa |
GenBank accession number | BC096336 |
Gene symbol | IDE |
Gene ID (NCBI) | 3416 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 IDE antibody CL488-67106 | Download protocol |
FC protocol for CL Plus 488 IDE antibody CL488-67106 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |