IgG Heavy chain Monoclonal antibody
Human IgG Heavy chain Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
1C6B4
Cat no : 67760-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | human plasma |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
67760-1-Ig targets Human IgG Heavy chain in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | nature protein 相同性解析による交差性が予測される生物種 |
| Full Name | Human IgG Heavy chain |
| Calculated molecular weight | 155 kDa |
| Observed molecular weight | ~50 kDa |
| Gene symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody detects the heavy chain of human IgG.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Human IgG Heavy chain antibody 67760-1-Ig | Download protocol |
| IHC protocol for Human IgG Heavy chain antibody 67760-1-Ig | Download protocol |
| IF protocol for Human IgG Heavy chain antibody 67760-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
