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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Histone-H3 Polyclonal antibody

Histone-H3 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17168

Synonyms

H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o, Histone-H3

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-17168

    FC experiment of HeLa using CL488-17168

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Histone-H3 (CL488-17168) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using CL488-17168

    FC experiment of HeLa using CL488-17168

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Histone-H3 (CL488-17168) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Histone-H3 Ready-To-Use IHC Kit. Histone-H3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17168-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-17168 is Histone-H3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone-H3 fusion protein Ag10644 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H3a
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC015544
Gene symbol HIST2H3A
Gene ID (NCBI) 333932
RRIDAB_2919145
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Histone-H3 antibody CL488-17168Download protocol
Standard Protocols
Click here to view our Standard Protocols