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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Heavy chain of Rabbit IgG Monoclonal antibody

Heavy chain of Rabbit IgG Monoclonal Antibody for IF, FC

Host / Isotype

Mouse / IgG1

Reactivity

rabbit

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C9A5

Cat no : CL647-66467

Synonyms

Heavy chain of Rabbit IgG

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL647-66467

    IF Staining of HeLa using CL647-66467

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using the CoraLite® Plus 647-conjugated version of this antibody, CL647-66467 (Heavy chain of Rabbit IgG antibody), at dilution of 1:200.

  • FC experiment of HepG2 using CL647-66467

    FC experiment of HepG2 using CL647-66467

    1X10^6 HepG2 cells were intracellularly stained with Anti-human TDP-43 recombinant antibody (80002-1-RR) and 0.2 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66467-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-66467 targets Heavy chain of Rabbit IgG in IF, FC applications and shows reactivity with rabbit samples.

Tested Reactivity rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Protein 相同性解析による交差性が予測される生物種
Full Name Heavy chain of Rabbit IgG
Gene symbol
Gene ID (NCBI)
RRIDAB_2883736
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody specifically recognizes the heavy chain of Rabbit IgG.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Heavy chain of Rabbit IgG antibody CL647-66467Download protocol
FC protocol for CL Plus 647 Heavy chain of Rabbit IgG antibody CL647-66467Download protocol
Standard Protocols
Click here to view our Standard Protocols