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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSPB11 Monoclonal antibody

HSPB11 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D11B3

Cat no : 68059-1-Ig

Synonyms

C1orf41, Heat shock protein beta 11, HSPB11, HSPCO34, ITF25, Placental protein 25, PP25

Validation Data Gallery

Applications:
  • WB analysis using 68059-1-Ig

    WB analysis using 68059-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68059-1-Ig (HSPB11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNCCIT cells, LNCaP cells, HeLa cells, HEK-293 cells, T-47D cells, Jurkat cells, K-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68059-1-Ig targets HSPB11 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HSPB11 fusion protein Ag8804 相同性解析による交差性が予測される生物種
Full Name heat shock protein family B (small), member 11
Calculated molecular weight 144 aa, 16 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC005245
Gene symbol HSPB11
Gene ID (NCBI) 51668
RRIDAB_2918800
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.

Protocols

Product Specific Protocols
WB protocol for HSPB11 antibody 68059-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols