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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HSF1 Monoclonal antibody

HSF1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F10A5

Cat no : CL488-67189

Synonyms

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-67189

    IF Staining of HepG2 using CL488-67189

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated HSF1 antibody (CL488-67189, Clone: 1F10A5 ) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSF1 Ready-To-Use IHC Kit. HSF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67189-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67189 targets HSF1 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSF1 fusion protein Ag29061 相同性解析による交差性が予測される生物種
Full Name heat shock transcription factor 1
Calculated molecular weight 529 aa, 57 kDa
Observed molecular weight 68-80 kDa
GenBank accession numberBC014638
Gene symbol HSF1
Gene ID (NCBI) 3297
RRIDAB_2919433
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HSF1 antibody CL488-67189Download protocol
Standard Protocols
Click here to view our Standard Protocols