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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated HNRNPD Monoclonal antibody

HNRNPD Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G9E6

Cat no : CL594-68236

Synonyms

AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-68236

    IF Staining of HepG2 using CL594-68236

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 HNRNPD antibody (CL594-68236, Clone: 2G9E6 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPD Ready-To-Use IHC Kit. HNRNPD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68236-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-68236 targets HNRNPD in IF applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HNRNPD fusion protein Ag3458 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
Calculated molecular weight 355 aa, 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC026015
Gene symbol HNRNPD
Gene ID (NCBI) 3184
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region (PMID:8578590,12704645). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).

Protocols

Product Specific Protocols
IF protocol for CL594 HNRNPD antibody CL594-68236Download protocol
Standard Protocols
Click here to view our Standard Protocols