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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDAC3 Recombinant antibody

HDAC3 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2N1

Cat no : 81211-1-RR

Synonyms

HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45

Validation Data Gallery

Applications:
  • WB analysis using 81211-1-RR

    WB analysis using 81211-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81211-1-RR (HDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 81211-1-RR

    IHC staining of human liver cancer using 81211-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human liver cancer using 81211-1-RR

    IHC staining of human liver cancer using 81211-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human thyroid cancer using 81211-1-RR

    IHC staining of human thyroid cancer using 81211-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human thyroid cancer using 81211-1-RR

    IHC staining of human thyroid cancer using 81211-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC3 antibody included

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MOLT-4 cells, Caco-2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman thyroid cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HDAC3 Ready-To-Use IHC Kit. HDAC3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

81211-1-RR targets HDAC3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HDAC3 fusion protein Ag0396 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 3
Calculated molecular weight 49 kDa
Observed molecular weight49 kDa
GenBank accession numberBC000614
Gene symbol HDAC3
Gene ID (NCBI) 8841
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene.

Protocols

Product Specific Protocols
WB protocol for HDAC3 antibody 81211-1-RRDownload protocol
IHC protocol for HDAC3 antibody 81211-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols