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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HBG1 Polyclonal antibody

HBG1 Polyclonal Antibody for WB, IP, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 25728-1-AP

Synonyms

Gamma 1 globin, Hb F Agamma, HBG1, HBGA, HBGR, Hemoglobin gamma 1 chain, Hemoglobin gamma A chain, Hemoglobin subunit gamma 1, hemoglobin, gamma A, HSGGL1, PRO2979

Validation Data Gallery

Applications:
  • WB analysis using 25728-1-AP

    WB analysis using 25728-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25728-1-AP (HBG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 25728-1-AP

    IP experiment of K-562 using 25728-1-AP

    IP result of anti-HBG1 (IP:25728-1-AP, 3ug; Detection:25728-1-AP 1:300) with K-562 cells lysate 2000ug.

  • FC experiment of K-562 using 25728-1-AP

    FC experiment of K-562 using 25728-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human HBG1 (25728-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inK-562 cells, human placenta tissue
Positive IP detected inK-562 cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy HBG1 Ready-To-Use IHC Kit. HBG1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

25728-1-AP targets HBG1 in WB, IP, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HBG1 fusion protein Ag22776 相同性解析による交差性が予測される生物種
Full Name hemoglobin, gamma A
Calculated molecular weight 147 aa, 16 kDa
Observed molecular weight 14-16 kDa
GenBank accession numberBC010913
Gene symbol HBG1
Gene ID (NCBI) 3047
RRIDAB_2880212
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HBG1 antibody 25728-1-APDownload protocol
IP protocol for HBG1 antibody 25728-1-APDownload protocol
FC protocol for HBG1 antibody 25728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cell Reports

FAM122A Inhibits Erythroid Differentiation through GATA1.

Authors - Jing Chen
View Article
humanIF

Front Pharmacol

Beneficial Effect of Edoxaban on Preventing Atrial Fibrillation and Coagulation by Reducing Inflammation via HBG1/HBD Biomarkers.

Authors - Chenguang Yang
View Article