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  • Featured Product
  • KD/KO Validated

Gelsolin Monoclonal antibody

Gelsolin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C4A2

Cat no : 66280-1-Ig

Synonyms

Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN

Validation Data Gallery

Applications:
  • WB analysis of U2OS using 66280-1-Ig

    WB analysis of U2OS using 66280-1-Ig

    U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66280-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • WB analysis of human plasma using 66280-1-Ig

    WB analysis of human plasma using 66280-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HT-29 using 66280-1-Ig

    WB analysis of HT-29 using 66280-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 66280-1-Ig

    WB analysis of A549 using 66280-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66280-1-Ig

    IHC staining of human colon using 66280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 66280-1-Ig

    IHC staining of human colon using 66280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human colon cancer using 66280-1-Ig

    IHC staining of human colon cancer using 66280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66280-1-Ig

    IHC staining of human colon cancer using 66280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman plasma tissue, U2OS cells, A549 cells, human plasma, HT-29 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66280-1-Ig targets Gelsolin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen Gelsolin fusion protein Ag17916 相同性解析による交差性が予測される生物種
Full Name gelsolin (amyloidosis, Finnish type)
Calculated molecular weight 782 aa, 86 kDa
Observed molecular weight 80-83 kDa
GenBank accession numberBC026033
Gene symbol GSN
Gene ID (NCBI) 2934
RRIDAB_2881663
Conjugate Unconjugated
Form Liquid
Purification Method Thiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease.

Protocols

Product Specific Protocols
WB protocol for Gelsolin antibody 66280-1-IgDownload protocol
IHC protocol for Gelsolin antibody 66280-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols