CoraLite®647-conjugated GST Tag Polyclonal antibody

GST Tag Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

FC

Conjugate

CoraLite®647 Fluorescent Dye

Cat no : CL647-10000

Synonyms

GST, Rabbit IgG control



Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL647-10000 targets GST Tag in FC applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 665 nm
Form Liquid
Purification Method Antigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Protocols

Product Specific Protocols
FC protocol for CL647 GST Tag antibody CL647-10000Download protocol
Standard Protocols
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