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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPT/ALT1 Monoclonal antibody

GPT/ALT1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, rat, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5F6B5

Cat no : 67531-1-Ig

Synonyms

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1

Validation Data Gallery

Applications:
  • WB analysis of rat liver using 67531-1-Ig

    WB analysis of rat liver using 67531-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67531-1-Ig

    WB analysis using 67531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 67531-1-Ig

    WB analysis of mouse liver using 67531-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig heart using 67531-1-Ig

    WB analysis of pig heart using 67531-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig liver using 67531-1-Ig

    WB analysis of pig liver using 67531-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67531-1-Ig

    IF Staining of HepG2 using 67531-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman milk, pig heart tissue, pig liver tissue, rat liver tissue, mouse liver tissue, rabbit heart tissue
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67531-1-Ig targets GPT/ALT1 in WB, IF, ELISA applications and shows reactivity with Human, Pig, rat, mouse samples.

Tested Reactivity Human, Pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPT/ALT1 fusion protein Ag10453 相同性解析による交差性が予測される生物種
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC018207
Gene symbol GPT
Gene ID (NCBI) 2875
RRIDAB_2882750
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

Protocols

Product Specific Protocols
WB protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
IF protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols