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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPKOW Monoclonal antibody

GPKOW Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G11C4

Cat no : 68338-1-Ig

Synonyms

G patch domain and KOW motifs, GPATC5, GPATCH5, GPKOW, Protein MOS2 homolog, Protein T54, T54

Validation Data Gallery

Applications:
  • WB analysis using 68338-1-Ig

    WB analysis using 68338-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68338-1-Ig (GPKOW antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Tested Applications

Positive WB detected inU2OS cells, LNcaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68338-1-Ig targets GPKOW in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPKOW fusion protein Ag6545 相同性解析による交差性が予測される生物種
Full Name G patch domain and KOW motifs
Calculated molecular weight 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC000397
Gene symbol GPKOW
Gene ID (NCBI) 27238
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPKOW is a RNA-binding protein involved in pre-mRNA splicing.It interacts directly with protein kinase A and protein kinase X and is also found associated with the spliceosome.

Protocols

Product Specific Protocols
WB protocol for GPKOW antibody 68338-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols