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- KD/KO Validated
GP73/GOLPH2 Monoclonal antibody
GP73/GOLPH2 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3D1F1
Cat no : 66331-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, HeLa cells, HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
66331-1-Ig targets GP73/GOLPH2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GP73/GOLPH2 fusion protein Ag7207 相同性解析による交差性が予測される生物種 |
| Full Name | golgi membrane protein 1 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 73 kDa |
| GenBank accession number | BC001740 |
| Gene symbol | GOLM1 |
| Gene ID (NCBI) | 51280 |
| RRID | AB_2881712 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GP73/GOLPH2 antibody 66331-1-Ig | Download protocol |
| IHC protocol for GP73/GOLPH2 antibody 66331-1-Ig | Download protocol |
| IF protocol for GP73/GOLPH2 antibody 66331-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol TCF11 Has a Potent Tumor-Repressing Effect Than Its Prototypic Nrf1α by Definition of Both Similar Yet Different Regulatory Profiles, With a Striking Disparity From Nrf2. | ||
Cell Rep Cholesterol suppresses GOLM1-dependent selective autophagy of RTKs in hepatocellular carcinoma.
| ||
Microbiol Spectr Associating bovine herpesvirus 1 envelope glycoprotein gD with activated phospho-PLC-γ1(S1248) |
