CoraLite® Plus 488-conjugated GOT2 Monoclonal antibody
GOT2 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig, chicken
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B6C6
Cat no : CL488-67738
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy GOT2 Ready-To-Use IHC Kit. GOT2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67738-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-67738 targets GOT2 in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig, chicken samples.
| Tested Reactivity | Human, mouse, rat, pig, chicken |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GOT2 fusion protein Ag6600 相同性解析による交差性が予測される生物種 |
| Full Name | glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 39-40 kDa |
| GenBank accession number | BC000525 |
| Gene symbol | GOT2 |
| Gene ID (NCBI) | 2806 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 GOT2 antibody CL488-67738 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
