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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated GNMT Monoclonal antibody

GNMT Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B5E3

Cat no : CL594-67294

Synonyms

glycine N methyltransferase, GNMT

Validation Data Gallery

Applications:
  • IF Staining of A431 using CL594-67294

    IF Staining of A431 using CL594-67294

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 GNMT antibody (CL594-67294, Clone: 1B5E3 ) at dilution of 1:200.

Tested Applications

Positive IF detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67294-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67294 targets GNMT in IF applications and shows reactivity with Human, Mouse, Pig, Rat samples.

Tested Reactivity Human, Mouse, Pig, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNMT fusion protein Ag4598 相同性解析による交差性が予測される生物種
Full Name glycine N-methyltransferase
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC032627
Gene symbol GNMT
Gene ID (NCBI) 27232
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

Protocols

Product Specific Protocols
IF protocol for CL594 GNMT antibody CL594-67294Download protocol
Standard Protocols
Click here to view our Standard Protocols