GNAI1 Monoclonal antibody
GNAI1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Mouse, Rat, Pig, Chicken
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G11H5
Cat no : 68266-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | rat brain tissue, pig brain tissue, chicken brain tissue, mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy GNAI1 Ready-To-Use IHC Kit. GNAI1 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
68266-1-Ig targets GNAI1 in WB, ELISA applications and shows reactivity with Mouse, Rat, Pig, Chicken samples.
| Tested Reactivity | Mouse, Rat, Pig, Chicken |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GNAI1 fusion protein Ag3290 相同性解析による交差性が予測される生物種 |
| Full Name | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 |
| Calculated molecular weight | 354 aa, 40 kDa |
| Observed molecular weight | 38-40 kDa |
| GenBank accession number | BC026326 |
| Gene symbol | GNAI1 |
| Gene ID (NCBI) | 2770 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAI1 antibody 68266-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
