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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GM130;GOLGA2 Recombinant antibody

GM130;GOLGA2 Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1M17

Cat no : 82705-8-RR

Synonyms

GM130, GM130 autoantigen, GOLGA2, Golgin 95, Golgin subfamily A member 2

Validation Data Gallery

Applications:
  • WB analysis using 82705-8-RR

    WB analysis using 82705-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82705-8-RR (GM130;GOLGA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82705-8-RR

    IF Staining of HeLa using 82705-8-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GM130;GOLGA2 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

82705-8-RR targets GM130;GOLGA2 in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GM130;GOLGA2 fusion protein Ag1848 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated molecular weight 111 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC014188
Gene symbol GOLGA2
Gene ID (NCBI) 2801
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Protocols

Product Specific Protocols
WB protocol for GM130;GOLGA2 antibody 82705-8-RRDownload protocol
IF protocol for GM130;GOLGA2 antibody 82705-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols