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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated GLUD1 Monoclonal antibody

GLUD1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4G10D3

Cat no : CL594-67026

Synonyms

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1

Validation Data Gallery

Applications:
  • IF Staining of human liver cancer using CL594-67026

    IF Staining of human liver cancer using CL594-67026

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594-conjugated GLUD1 antibody (CL594-67026, Clone: 4G10D3 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL594-67026

    IF Staining of human liver cancer using CL594-67026

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594-conjugated GLUD1 antibody (CL594-67026, Clone: 4G10D3 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy GLUD1 Ready-To-Use IHC Kit. GLUD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67026-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67026 targets GLUD1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GLUD1 fusion protein Ag6179 相同性解析による交差性が予測される生物種
Full Name glutamate dehydrogenase 1
Calculated molecular weight 61 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC040132
Gene symbol GLUD1
Gene ID (NCBI) 2746
RRIDAB_2920074
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

Protocols

Product Specific Protocols
IF protocol for CL594 GLUD1 antibody CL594-67026Download protocol
Standard Protocols
Click here to view our Standard Protocols