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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GIT2 Monoclonal antibody

GIT2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D9C5

Cat no : 67858-1-Ig

Synonyms

ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148

Validation Data Gallery

Applications:
  • WB analysis using 67858-1-Ig

    WB analysis using 67858-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of K-562 using 67858-1-Ig

    WB analysis of K-562 using 67858-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67858-1-Ig

    WB analysis of HSC-T6 using 67858-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 67858-1-Ig

    IHC staining of human prostate cancer using 67858-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67858-1-Ig (GIT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, K-562 cells, HSC-T6 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67858-1-Ig targets GIT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GIT2 fusion protein Ag0321 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor kinase interacting ArfGAP 2
Calculated molecular weight 95 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC001379
Gene symbol GIT2
Gene ID (NCBI) 9815
RRIDAB_2918616
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GIT2 antibody 67858-1-IgDownload protocol
IHC protocol for GIT2 antibody 67858-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols