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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated GATA2 Monoclonal antibody

GATA2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B5A1

Cat no : CL488-67722

Synonyms

FLJ45948, GATA binding protein 2, GATA2, NFE1B

Validation Data Gallery

Applications:
  • FC experiment of K-562 using CL488-67722

    FC experiment of K-562 using CL488-67722

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GATA2 (CL488-67722, Clone:2B5A1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy GATA2 Ready-To-Use IHC Kit. GATA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67722-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67722 targets GATA2 in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA2 fusion protein Ag1557 相同性解析による交差性が予測される生物種
Full Name GATA binding protein 2
Observed molecular weight50 kDa
GenBank accession numberBC018988
Gene symbol GATA2
Gene ID (NCBI) 2624
RRIDAB_2919527
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 GATA2 antibody CL488-67722Download protocol
Standard Protocols
Click here to view our Standard Protocols