Frizzled 9 Monoclonal antibody
Frizzled 9 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1G9G4
Cat no : 67023-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HUVEC cells, NCI-H1299 cells, fetal human brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HEK-293 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
Product Information
67023-1-Ig targets Frizzled 9 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Frizzled 9 fusion protein Ag4971 相同性解析による交差性が予測される生物種 |
| Full Name | frizzled homolog 9 (Drosophila) |
| Calculated molecular weight | 591 aa, 64 kDa |
| Observed molecular weight | 64-70 kDa |
| GenBank accession number | BC026333 |
| Gene symbol | FZD9 |
| Gene ID (NCBI) | 8326 |
| RRID | AB_2882338 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Frizzled 9 antibody 67023-1-Ig | Download protocol |
| IHC protocol for Frizzled 9 antibody 67023-1-Ig | Download protocol |
| IF protocol for Frizzled 9 antibody 67023-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Susanne (Verified Customer) (04-17-2023) |
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