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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FXYD1 Monoclonal antibody

FXYD1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C10G9

Cat no : CL488-67150

Synonyms

FXYD1, Phospholemman, PLM

Validation Data Gallery

Applications:
  • FC experiment of C2C12 using CL488-67150

    FC experiment of C2C12 using CL488-67150

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FXYD1 (CL488-67150, Clone:2C10G9) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy FXYD1 Ready-To-Use IHC Kit. FXYD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67150-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67150 targets FXYD1 in FC (Intra) applications and shows reactivity with Human, Pig, mouse samples.

Tested Reactivity Human, Pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FXYD1 fusion protein Ag25307 相同性解析による交差性が予測される生物種
Full Name FXYD domain containing ion transport regulator 1
Calculated molecular weight 92 aa, 10 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC032800
Gene symbol FXYD1
Gene ID (NCBI) 5348
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 FXYD1 antibody CL488-67150Download protocol
Standard Protocols
Click here to view our Standard Protocols