FH Recombinant antibody
FH Recombinant Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat, Mouse
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
3K16
Cat no : 82508-1-RR
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, A431 cells, L02 cells, mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
82508-1-RR targets FH in WB, IP, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
| Tested Reactivity | Human, Rat, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | FH fusion protein Ag1934 相同性解析による交差性が予測される生物種 |
| Full Name | fumarate hydratase |
| Calculated molecular weight | 510 aa, 55 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC003108 |
| Gene symbol | FH |
| Gene ID (NCBI) | 2271 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FH antibody 82508-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
