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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FH Monoclonal antibody

FH Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9C8

Cat no : 68162-1-Ig

Synonyms

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1

Validation Data Gallery

Applications:
  • WB analysis using 68162-1-Ig

    WB analysis using 68162-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68162-1-Ig (FH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Tested Applications

Positive WB detected inA431 cells, LNCaP cells, HepG2 cells, HEK-293 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68162-1-Ig targets FH in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FH fusion protein Ag30254 相同性解析による交差性が予測される生物種
Full Name fumarate hydratase
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC003108
Gene symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for FH antibody 68162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols