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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FBL Polyclonal antibody

FBL Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16021

Synonyms

FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-16021

    IF Staining of HeLa using CL488-16021

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FBL antibody (CL488-16021) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IF Staining of HepG2 using CL488-16021

    IF Staining of HepG2 using CL488-16021

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 FBL antibody (CL488-16021) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FBL Ready-To-Use IHC Kit. FBL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16021-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-16021 targets FBL in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBL fusion protein Ag8841 相同性解析による交差性が予測される生物種
Full Name fibrillarin
Calculated molecular weight 321 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC019260
Gene symbol FBL
Gene ID (NCBI) 2091
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FBL antibody CL488-16021Download protocol
Standard Protocols
Click here to view our Standard Protocols