Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 16021-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16021-1-AP (Fibrillarin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 16021-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16021-1-AP (Fibrillarin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16021-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (Fibrillarin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A375 using 16021-1-AP
IP result of anti-Fibrillarin (IP:16021-1-AP, 4ug; Detection:16021-1-AP 1:500) with A375 cells lysate 3600ug.
IHC staining of mouse brain using 16021-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16021-1-AP (Fibrillarin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16021-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Fibrillarin antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 16021-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Fibrillarin antibody (16021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 16021-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Fibrillarin antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 16021-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Fibrillarin (16021-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

Fibrillarin Polyclonal antibody

Fibrillarin Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16021-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, RIP, ELISA

Synonyms

FBL, EC:2.1.1.-, FIB, FIB1, FLRN

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (35)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A375 cells, HCT 116 cells, mouse liver tissue, HeLa cells, Jurkat cells
Positive IP detected in	A375 cells
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 22 publications below
IF	See 11 publications below
IP	See 1 publications below
RIP	See 4 publications below

Product Information

16021-1-AP targets Fibrillarin in WB, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8841 Product name: Recombinant human FBL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-321 aa of BC019260 Sequence: MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN 相同性解析による交差性が予測される生物種
Full Name	fibrillarin
Calculated molecular weight	321 aa, 34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC019260
Gene Symbol	FBL
Gene ID (NCBI)	2091
RRID	AB_2105788
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22087
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

Protocols

Product Specific Protocols
FC protocol for Fibrillarin antibody 16021-1-AP	Download protocol
IF protocol for Fibrillarin antibody 16021-1-AP	Download protocol
IHC protocol for Fibrillarin antibody 16021-1-AP	Download protocol
IP protocol for Fibrillarin antibody 16021-1-AP	Download protocol
WB protocol for Fibrillarin antibody 16021-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Oxidative cyclization reagents reveal tryptophan cation-π interactions Authors - Xiao Xie View Article
human	WB,RIP,IF	Mol Cell lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing Authors - Yu-Meng Sun View Article
human	WB	Mol Cell LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice. Authors - Xin Wang KD Validated View Article
human	WB	Nucleic Acids Res GPATCH4 functions as a regulator of nucleolar R-loops in hepatocellular carcinoma cells Authors - Yi-Ming Zhao View Article
human	WB	Breast Cancer Res U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression Authors - Wenrong Zhang View Article
human	WB,IF	Cell Biol Toxicol SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells Authors - Jingyi Cui View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16021-1-AP

WB analysis using 16021-1-AP

WB analysis of mouse liver using 16021-1-AP

IP experiment of A375 using 16021-1-AP

IHC staining of mouse brain using 16021-1-AP

IF Staining of HepG2 using 16021-1-AP

IF Staining of HepG2 using 16021-1-AP

IF Staining of HepG2 using 16021-1-AP

FC experiment of HepG2 using 16021-1-AP

Fibrillarin Polyclonal antibody

Fibrillarin Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Oxidative cyclization reagents reveal tryptophan cation-π interactions

lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing

LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.

GPATCH4 functions as a regulator of nucleolar R-loops in hepatocellular carcinoma cells

U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression

SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells