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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Egfr Polyclonal antibody

Egfr Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30847-1-AP

Synonyms

9030024J15Rik, AI552599, Egfr, Erbb, Errp, wa 2, wa2, Wa5

Validation Data Gallery

Applications:
  • WB analysis using 30847-1-AP

    WB analysis using 30847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30847-1-AP (Egfr antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30847-1-AP

    IF Staining of A431 using 30847-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Egfr antibody (30847-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, mouse liver tissue
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

30847-1-AP targets Egfr in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Egfr fusion protein Ag33621 相同性解析による交差性が予測される生物種
Full Name epidermal growth factor receptor
Calculated molecular weight135 kDa
Observed molecular weight140-180 kDa
GenBank accession numberNM_207655
Gene symbol Egfr
Gene ID (NCBI) 13649
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The epidermal growth factor receptor (EGFR) is a member of the ErbB family of receptor tyrosine kinases (RTKs). Binding of EGFR to epidermal growth factor family ligands triggers a signaling cascade through allosteric activation of epidermal growth factor receptor kinase and trans-autophosphorylation of key tyrosine residues in the tail of the cytoplasmic receptor (PMID: 24691965). Due to phosphorylation, the apparent molecular weight is higher than the calculated molecular weight of 140 kDa. EFGR has been implicated in learning and memory enhancement and pain signaling in mammals (PMID: 20639532 and 35131940).

Protocols

Product Specific Protocols
WB protocol for Egfr antibody 30847-1-APDownload protocol
IF protocol for Egfr antibody 30847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols