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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EXO1 Monoclonal antibody

EXO1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D11D1

Cat no : 68536-1-Ig

Synonyms

EXO1, EXOI, exonuclease 1, Exonuclease I, HEX1, hExo1, hExoI

Validation Data Gallery

Applications:
  • WB analysis using 68536-1-Ig

    WB analysis using 68536-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68536-1-Ig (EXO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells, HEK-293T cells, MOLT-4 cells, TF-1 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68536-1-Ig targets EXO1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EXO1 fusion protein Ag9253 相同性解析による交差性が予測される生物種
Full Name exonuclease 1
Calculated molecular weight 94 kDa
Observed molecular weight 115-120 kDa
GenBank accession numberBC007491
Gene symbol EXO1
Gene ID (NCBI) 9156
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.

Protocols

Product Specific Protocols
WB protocol for EXO1 antibody 68536-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols