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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERCC5 Monoclonal antibody

ERCC5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G2C9

Cat no : 67055-1-Ig

Synonyms

COFS3, ERCC5, ERCM2, UVDR, XPG, XPGC

Validation Data Gallery

Applications:
  • WB analysis using 67055-1-Ig

    WB analysis using 67055-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Daudi using 67055-1-Ig

    WB analysis of Daudi using 67055-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Ramos using 67055-1-Ig

    WB analysis of Ramos using 67055-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 67055-1-Ig

    WB analysis of COLO 320 using 67055-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHT-29 cells, COLO 320 cells, Daudi cells, Ramos cells, HeLa cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67055-1-Ig targets ERCC5 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERCC5 fusion protein Ag28561 相同性解析による交差性が予測される生物種
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 5
Calculated molecular weight 1186 aa, 133 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC031522
Gene symbol ERCC5
Gene ID (NCBI) 2073
RRIDAB_2882366
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.

Protocols

Product Specific Protocols
WB protocol for ERCC5 antibody 67055-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Pharmacol

Triptolide enhances carboplatin-induced apoptosis by inhibiting nucleotide excision repair (NER) activity in melanoma

Authors - Geng Wang
View Article