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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ELAVL4 Monoclonal antibody

ELAVL4 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse, rabbit, pig

Applications

WB, IF, FC, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1B1F5

Cat no : 67835-1-Ig

Synonyms

ELAV like protein 4, ELAVL4, Hu antigen D, HUD, PNEM

Validation Data Gallery

Applications:
  • WB analysis of U2OS using 67835-1-Ig

    WB analysis of U2OS using 67835-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 67835-1-Ig

    WB analysis of SH-SY5Y using 67835-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67835-1-Ig

    WB analysis using 67835-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67835-1-Ig

    IHC staining of mouse brain using 67835-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67835-1-Ig

    IHC staining of mouse brain using 67835-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 67835-1-Ig

    IF Staining of U2OS using 67835-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ELAVL4 antibody (67835-1-Ig, Clone: 1B1F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of U2OS using 67835-1-Ig

    FC experiment of U2OS using 67835-1-Ig

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ELAVL4 (67835-1-Ig, Clone:1B1F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inpig brain tissue, U2OS cells, SH-SY5Y cells, rat brain tissue, mouse brain tissue, rabbit brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells
Positive FC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

CoIPSee 1 publications below

Product Information

67835-1-Ig targets ELAVL4 in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with Human, rat, mouse, rabbit, pig samples.

Tested Reactivity Human, rat, mouse, rabbit, pig
Cited Reactivity mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ELAVL4 fusion protein Ag3678 相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
Calculated molecular weight 380 aa, 42 kDa
Observed molecular weight32-40 kDa
GenBank accession numberBC036071
Gene symbol ELAVL4
Gene ID (NCBI) 1996
RRIDAB_2918597
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

Protocols

Product Specific Protocols
WB protocol for ELAVL4 antibody 67835-1-IgDownload protocol
IHC protocol for ELAVL4 antibody 67835-1-IgDownload protocol
IF protocol for ELAVL4 antibody 67835-1-IgDownload protocol
FC protocol for ELAVL4 antibody 67835-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseCoIP

Mol Neurobiol

NCBP1 Improves Cognitive Function in Mice by Reducing Oxidative Stress, Neuronal Loss, and Glial Activation After Status Epilepticus

Authors - Xiaoying Gao
View Article