CoraLite® Plus 488-conjugated ELAVL4 Monoclonal antibody
ELAVL4 Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rat, mouse, rabbit, pig
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B1F5
Cat no : CL488-67835
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | mouse brain tissue, U2OS cells |
Positive FC detected in | U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-67835 targets ELAVL4 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse, rabbit, pig samples.
Tested Reactivity | Human, rat, mouse, rabbit, pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | ELAVL4 fusion protein Ag3678 相同性解析による交差性が予測される生物種 |
Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) |
Calculated molecular weight | 380 aa, 42 kDa |
Observed molecular weight | 32-40 kDa |
GenBank accession number | BC036071 |
Gene symbol | ELAVL4 |
Gene ID (NCBI) | 1996 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 ELAVL4 antibody CL488-67835 | Download protocol |
FC protocol for CL Plus 488 ELAVL4 antibody CL488-67835 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |