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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF4A2 Monoclonal antibody

EIF4A2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D11E11

Cat no : 67799-1-Ig

Synonyms

BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F

Validation Data Gallery

Applications:
  • WB analysis using 67799-1-Ig

    WB analysis using 67799-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67799-1-Ig (EIF4A2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67799-1-Ig

    IF Staining of HEK-293 using 67799-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4A2 Ready-To-Use IHC Kit. EIF4A2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67799-1-Ig targets EIF4A2 in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EIF4A2 fusion protein Ag9085 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4A, isoform 2
Calculated molecular weight 408 aa, 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC012547
Gene symbol EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2918563
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

Protocols

Product Specific Protocols
WB protocol for EIF4A2 antibody 67799-1-IgDownload protocol
IF protocol for EIF4A2 antibody 67799-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols